Custom Peptide Synthesis

Custom Peptide Synthesis Services

Need a peptide sequence that is not in our standard catalog? NXPeptides offers custom peptide synthesis services for researchers who require specific sequences, modifications, or quantities tailored to their experimental needs. Whether you need a short linear peptide, a complex modified sequence, or bulk quantities for ongoing studies, our synthesis team can help.

What We Can Synthesize

Our custom synthesis capabilities include:

• Standard linear peptides: Up to 100 amino acids in length, using standard Fmoc solid-phase peptide synthesis
• Modified peptides: N-terminal acetylation, C-terminal amidation, phosphorylation, biotinylation, fluorescent labeling, PEGylation, and many other modifications
• Cyclic peptides: Head-to-tail, disulfide bridge, lactam bridge, and other cyclization chemistries
• Peptide conjugates: Peptides conjugated to carrier proteins, fatty acids, or other molecules
• Isotope-labeled peptides: For mass spectrometry-based quantitation (AQUA peptides, SILAC standards)
• D-amino acid peptides: Sequences incorporating D-enantiomers for protease resistance studies
• Peptide libraries: Sets of related sequences for SAR studies or screening applications

Purity Options

We offer several purity grades for custom peptides to match your application requirements and budget:

• Crude (desalted): Suitable for initial screening and qualitative experiments where high purity is not critical
• Greater than 90%: Appropriate for many general research applications
• Greater than 95%: Good for most biological assays and immunization protocols
• Greater than 98%: Our standard high-purity grade, suitable for quantitative studies
• Greater than 99%: Ultra-high purity for the most demanding applications

All custom peptides undergo the same rigorous quality control testing as our catalog products, including HPLC analysis and mass spectrometry. A detailed Certificate of Analysis is provided with every order.

How to Request a Custom Synthesis Quote

Getting a quote for custom peptide synthesis is straightforward:

1. Send us your sequence: Email support@nxpeptides.com with the amino acid sequence (one-letter or three-letter code), any desired modifications, target purity, and quantity needed.
2. We review and respond: Our synthesis team will review your request and provide a quote within 24 to 48 hours. For complex sequences or modifications, we may reach out with questions to make sure we understand your needs correctly.
3. Approve and we begin: Once you approve the quote, synthesis begins. We keep you updated on progress throughout the process.
4. QC and delivery: After synthesis and purification, the peptide undergoes full QC testing. Once it passes, we ship it to you via our temperature-controlled shipping process.

You can also submit your request through our Contact page form.

Turnaround Times

Typical turnaround times from order approval to shipment:

• Standard linear peptides (up to 30 aa, greater than 95% purity): 2 to 3 weeks
• Longer peptides (30 to 60 aa): 3 to 4 weeks
• Complex modifications or cyclization: 3 to 5 weeks
• Difficult sequences (aggregation-prone, very hydrophobic): 4 to 6 weeks
• Rush service: Available for an additional fee. Contact us for details.

These are estimates and actual timelines may vary depending on synthesis difficulty. If you have a hard deadline, let us know upfront so we can plan accordingly.

Pricing

Custom peptide pricing depends on several factors:

• Sequence length (longer peptides are more expensive to synthesize)
• Target purity (higher purity requires more purification)
• Quantity (per-unit cost decreases with larger quantities)
• Modifications (some modifications add to the cost)
• Difficulty (aggregation-prone or complex sequences may require special techniques)

We are competitive on pricing and work with researchers at all budget levels. For the best pricing on large or recurring orders, ask about volume discounts when you request your quote.

Quality Guarantee

Every custom peptide from NXPeptides comes with:

• A full Certificate of Analysis with HPLC purity and mass spectrometry data
• Free replacement or refund if the peptide does not meet agreed-upon specifications
• Technical support from our team to help with reconstitution, storage, and handling

Learn more about our quality standards on the Quality Assurance page.

Shipping Custom Orders

Custom peptide orders ship the same way as all NXPeptides products: free worldwide delivery with temperature-controlled packaging, discrete labeling, and tracking provided. Domestic orders typically arrive within 3 to 5 business days after shipment; international orders within 5 to 10 business days. Track your order anytime at our Order Tracker.

Frequently Asked Questions About Custom Synthesis

Can you synthesize peptides longer than 100 amino acids?
Sequences above 100 residues are challenging by standard SPPS. In some cases, we can use native chemical ligation or fragment condensation. Contact us to discuss feasibility for your specific sequence.

What if the synthesis fails?
Some sequences are inherently difficult to synthesize. If we are unable to produce your peptide to specification, you will not be charged. We will communicate any issues promptly and discuss alternatives.

Can you incorporate non-natural amino acids?
Yes, we have access to a wide range of non-natural and unusual amino acids. Include these in your sequence description when requesting a quote.

For more answers, visit our FAQ page or get in touch at support@nxpeptides.com.

All NXPeptides products, including custom synthesized peptides, are for research use only. Not for human consumption.